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LGC Group video
Приєднався 18 лип 2013
LGC is an international leader in the laboratory services, measurement standards, reference materials, genomics and proficiency testing marketplaces.
We work with our customers in pharmaceuticals and biotechnology, food, agricultural biotechnology, sports and many other sectors, as well as with the government to achieve excellence in investigative, diagnostic and measurement science.
We help customers conform to international statutory and regulatory standards.
Science is at the heart of all we do - for a safer world.
We work with our customers in pharmaceuticals and biotechnology, food, agricultural biotechnology, sports and many other sectors, as well as with the government to achieve excellence in investigative, diagnostic and measurement science.
We help customers conform to international statutory and regulatory standards.
Science is at the heart of all we do - for a safer world.
Відео
An introduction to alternative proteins
Переглядів 172Місяць тому
Current global food production practices are reported to be associated with production of high levels of greenhouse gas emissions. A recent report by the UK Committee on Climate Change indicates that at least a 20% reduction in the consumption of animal and dairy products would be necessary for the UK to achieve its target net-zero carbon emissions by 2050. Switching to sustainable, alternative...
sbeadex Lighting - Watch live DNA purification in under 5 minutes
Переглядів 352Місяць тому
It’s true - sbeadex™ Lightning can deliver purified DNA in typically under 5 minutes from lysate to eluate. In this real-life demonstration, Dr Heiko Hauser, Director of Research and Development, Nucleic Acid Purification, at LGC Biosearch Technologies, extracts high-volume, high-yield, highly-purified DNA from canola seeds. The purified DNA is suitable for a range of downstream applications in...
Probes for the quantification of specific target sequences
Переглядів 1076 місяців тому
BHQ™ Probes are traditional, linear, dual-labeled FRET probes with a fluorophore and a quencher covalently linked to the 5’ and 3’ ends, respectively. They are typically 20 to 30 bases in length. The 5' exonuclease activity of Taq polymerase triggers the release of a fluorescent dye upon hybridization of the probe with its complementary sequence, resulting in the cleavage of the dye. BHQ Probes...
MGB probes for any application
Переглядів 1656 місяців тому
MGB probes by LGC Biosearch Technologies’ are fluorogenic probes with a 3′ non-fluorescent quencher conjugated to a MGB moiety. High quality, cost effective MGB probes are available to order online - with more formulation options and flexibility than other oligo suppliers. Learn more: www.biosearchtech.com/products/pcr-reagents-kits-and-instruments/pcr-probes-and-assays/custom-dna-probes-for-qp...
Why use Scorpions Primers?
Переглядів 2 тис.7 місяців тому
Scorpions™ Primers combine primer and probe into a single bifunctional molecule. They contain a primer sequence at the 3′ end and a hairpin loop structure at the 5′ end. Like molecular beacons, the hairpin brings the reporter and quencher into proximity and the loop contains a sequence complementary to the target. Learn more: www.biosearchtech.com/products/pcr-reagents-kits-and-instruments/pcr-...
Try Molecular Beacons for your multiplex assay
Переглядів 1,1 тис.7 місяців тому
Molecular Beacon probes are dual-labelled probes that form a stem-loop (hairpin) structure, bringing the reporter and quencher into proximity. The loop region contains the sequence that hybridises to the target sequence, while the complementary sequences at both ends of the probe form the stem. These probes generate fluorescence under non-hydrolytic conditions via hybridisation to the target. L...
Custom LNA oligos for commercial use
Переглядів 1717 місяців тому
Improve assay sensitivity and specificity with Locked Nucleic Acid (LNA) probes for qPCR and SNP genotyping. LNAs enable a shorter probe, resulting in higher quenching efficiency, greater signal-to-noise ratio and enhanced detection of A-T rich sequences. Available as a custom oligo or primer with up to 20 LNA base insertions. Learn more: www.biosearchtech.com/products/pcr-reagents-kits-and-ins...
Detect more difficult targets with BHQplus Probes
Переглядів 917 місяців тому
BHQplus™ Probes from LGC Biosearch Technologies are short dual-labeled hydrolysis probes available for qPCR and SNP genotyping. This probe type features a 3' Black Hole Quencher™ (BHQ) and 5' fluorescent dye with the special BHQplus duplex stabilizing chemistry to improve specificity and improve mismatch discrimination. BHQplus Probes use altered C and T nucleotides to steady the probe-target d...
A probe for lower background fluorescence and enhanced assay sensitivity
Переглядів 427 місяців тому
When a longer probe design is necessary, such as achieving a desired melting temperature (Tm) in an AT-rich target region, the increased distance between the fluorophore and quencher in standard end-labeled probes may result in reduced quenching efficiency. In double-quenched BHQnova Probes the nova quencher is closer to the fluorophore which serves to enhance quenching efficacy. Learn more: ww...
sbeadex Lightning, 5-minute purification transforms DNA extraction times
Переглядів 8647 місяців тому
LGC Biosearch Technologies™ has launched a nucleic acid extraction method, sbeadex™ Lightning. This product rapidly delivers high-purity DNA in high-yields, typically achieving results in five minutes - from lysate to eluate - for both plant and animal samples. This magnetic bead technology significantly reduces the extraction workflow from a 5-step process to a simple 3-step method of bind, wa...
Introduction to LGC
Переглядів 5887 місяців тому
Driving science forward. LGC is a global life science tools company with a broad portfolio. We work closely with our diverse customers to drive science forward and find solutions that diagnose, heal, and help feed our growing population. Together we solve complex challenges such as managing global pandemics, pioneering precision medicine, improving agriculture outputs, and ensuring the safety o...
Databases for non-targeted food authentication analysis
Переглядів 678 місяців тому
An eSeminar from the UK's National Measurement Laboratory hosted at LGC. This eSeminar was based on the Food Integrity Scientific Opinion on databases (doi.org/10.1016/j.tifs.2019. 02.019), and introduces the viewer to the subject of the design, structure, and limitations of databases for non-targeted multianalyte methods. Examples of relational databases will be provided, and common challenges...
IR-MS for food authentication analysis
Переглядів 61711 місяців тому
An eSeminar on food authentication analysis, from the UK's National Measurement Laboratory hosted at LGC. This eSeminar, jointly authored by Simon Kelly (IAEA) and Philip Dunn (LGC), will introduce the viewer to the application of isotope ratio mass spectrometry (IRMS) for use with food authenticity, and quality testing. Detail of both the theory and application of the technique will be provide...
KASP Assay mix and KASP Master mix
Переглядів 1,2 тис.Рік тому
KASP is a PCR-based genotyping chemistry suitable for genotyping SNPs and InDels. KASP has been used to genotype millions of samples worldwide. If you have you ever wondered “How does KASP work?”, this series of three videos explains the KASP reagents, the genotyping reaction and how the data is analysed. This first video is about the components that go into a typical KASP genotyping reaction. ...
KASP by the numbers: unmatched experience
Переглядів 144Рік тому
KASP by the numbers: unmatched experience
LGC Biosearch Technologies simplifies online business for genomic analysis tools
Переглядів 5442 роки тому
LGC Biosearch Technologies simplifies online business for genomic analysis tools
Guidance for rapid identification of the Omicron variant
Переглядів 2552 роки тому
Guidance for rapid identification of the Omicron variant
Detecting antibodies against SARS CoV 2 using remote home sampling
Переглядів 8473 роки тому
Detecting antibodies against SARS CoV 2 using remote home sampling
Brian Brookman Proficiency testing / external quality assessment for SARS-CoV-2 virus testing by PCR
Переглядів 6584 роки тому
Brian Brookman Proficiency testing / external quality assessment for SARS-CoV-2 virus testing by PCR
Russell Garlick - LGC COVID Testing
Переглядів 1,3 тис.4 роки тому
Russell Garlick - LGC COVID Testing
Jim Huggett - The importance of standardisation of diagnostic measurements in the COVID-19 pandemic
Переглядів 7334 роки тому
Jim Huggett - The importance of standardisation of diagnostic measurements in the COVID-19 pandemic
Ultra-sensitive Biomarker Detection: Taking new technologies into the new decade
Переглядів 5334 роки тому
Ultra-sensitive Biomarker Detection: Taking new technologies into the new decade
Our impact: Improving productivity of palm plants
Переглядів 7534 роки тому
Our impact: Improving productivity of palm plants
Elissa Mount
Emmanuelle Courts
Mmm 😋, I can't wait to eat mealworms and crickets.
the probe is designed to only bind to certain sequences, for example it can be designed to only bind to a DNA sequence with a certain missense mutation and activate the fluorescence
👎
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LGC company is looking
Fascinating, this is cutting edge UK high tech!
Thank you very much 🙏
super informative, thanks!
great video thanks!
What purity of isotope for being Internal stanadard In determination by LC-MS/MS % purity from qNMR or hplc
2022, February 1st, look at the mess out country has become due to this fentynl. Heroin was one thing. It was actually pretty darn safe. It's long gone. Here in Detroit it's been gone for 5 years, only fentynl since then. It has no euphoria, none of the feel good of heroin, just a nasty drug that either puts you to sleep or if it don't you really don't feel it..... or the 3rd option- you die. It seems to be not only 50x stronger than heroin.....but 50x harder to quit. Horrid. 21 of my 40 years stuck on IV heroin and fentynl. My brain just doesn't work without it anymore.
At the very least with methadone, you always have a wake up, rarely go sick, and you can eventually work up to having to go only once a month. Street dope, has to many negative longterm outcomes. Mitigate those issues & you can have a more sane healthy life.
Next video ua-cam.com/video/GJbM7UbE7ZI/v-deo.html
Muchas gracias por la información!!!!!!!!
Well described the process
I wonder if the probe-oligonucleotide will be denatured (separated from quencher) during the first round of PCR and emit signal ? or the probe has to bind on the template DNA to emit signal ?
From my perspective, the probe, can emit signal in the first place, but it is inhibited by quencher. Therefore, in the first round the X/Y complement haven't been created yet. The probe will eventually end up with the quencher, but in the third round, the complement is created and the probe bind to it, in the other words, the probe is permanently separated from sequence which allow it to emit the signal.
I think the signal is only read at the end of the PCR-reaction. At this stage, when the temperature is lowered, the unbound reporters will bind again with the quenchers. Therefore no signal is emitted when the results are interpretted.
5:35 The Battle of Fromelles wasn't the first major battle fought by Australian troops in the First World War. Gallipoli was. Fromelles was their first major battle on the Western Front, however. Apart from that, very interesting video and good to see the important work that you're doing.
how to interpret the graph ?
Thanks a lot! your video is amazing
Need ful
nice explanation
Ghanta
Is this equipment safe to extract DNA from COVID-19 positive blood samples and other high-risk samples given the nature of the centrifuge and decantation? Can these processes cause aerosol formation?
Carl Nelson
may i know the HS code for this device ? my colleague will buy but need to make sure the customs rules accordingly
What does (the blue box and) the reverse primer do in this scenario?
me neither know what does allelle 2, reverse primer is clair
My Great Great Grandad, Private Harold Bates, (Royal 2/7 Warwickshire Regiment was killed in this battle and his body was never found. It's most likely, thanks to my father's investigations, that he is in an unmarked grave, at Cabaret Rouge. RIP Harold.
Can we buy stuff like ketamine for animals (cats)
Asking for a friend 😂
@@mikenaughton not really ! for me hehe
*saving you money*
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somebody explain this shit to me
I cannot understand anything, it will be great to have subtitles
Do anybody know why KASP markers are longer than usual markers ? Thanks
Nice technology. But... what is the song name?
It's literally in the end credits...
beautiful!!! :D ... thanks a lot for the animation... great explanation :D
After genotyping all these SNPs: How to assess the effect size of each SNP in disease susceptibility? Thank you
In order to begin to identify the effect of a given SNP in a population, you would need to at least have the disease susceptibility data, and the genotypic data for the same individuals, and then run a correlation between the susceptibility and the given SNP states. Look up 'genome wide association studies' (GWAS), and 'Quantitative Trait Loci' (QTL) mapping studies in order to see some of the common methods used for this. Bear in mind, the effect is the difference between the average with 1 allele and the average with the other allele. A SNP is only relevant for diagnosing susceptibility in a population if the effect is both statistically significant, and quantitatively sizable. Granted, if the SNP itself is causative for a specific phenotype, you'd want to study the effect with as little background noise as possible, and looking at back-crossed mutants when compared to wild-type individuals might then be optimal.
No sound?